Laboratory diagnosis of fungal infections pdf material may be challenged and removed. Dermatophytes produce an infection commonly known as ringworm or tinea. It appears anywhere on the skin and produces red or gray, scaly patches of itchy skin.
Deeper infections may be discoloured, ulcerative and purulent. The KOH test for fungus is conducted on an outpatient basis and patients do not need to prepare in advance. If fungal cultures are required, the test is performed by a technologist who specializes in microbiology. Collection: Skin, nail, or hair samples are collected from the infected area on the patient. For skin samples, a scalpel or edge of a glass slide is used to gently scrape skin scales from the infected area. For hair samples, a forceps is used to remove hair shafts and follicles from the infected site. If the test is being sent to a laboratory, the scrapings are placed in a sterile covered container.
The slide is left to stand until clear, normally between five and fifteen minutes, in order to dissolve skin cells, hair, and debris. The slide is gently heated to speed up the action of the KOH. Place the slide under a microscope to read. Under the microscope Tinea versicolor is recognized by curved hyphae and round yeast forms that give it a spaghetti-and-meatball appearance. Yeast cells appear round or oval and budding forms may be seen.
Dermatophytes or yeast seen on a KOH test indicate the person has a fungal infection. Follow-up tests are usually unnecessary. The skin may be sore after the test because of the tissue being scraped off the top of the surface of the skin. Cost containment: freestanding emergency centers and the emergency department”. This page was last edited on 3 December 2017, at 03:54. In old people and young children, the symptoms are not always as clear. Women get urinary tract infections more often than men.
Half of women have an infection at some point in their lives. It is common to have repeated infections. Sometimes a person who had a bladder infection will get a kidney infection. Kidney infection also can be caused by bacteria in the blood. Diagnosis in young healthy women can be based on symptoms alone. Sometimes, the urine needs to be tested. Antibiotics are used to treat simple cases of urinary tract infections.