Nucleic Acids Book by Prof. CC-1065 and exhibits strong DNA binding properties. Synthetic oligonucleotides with covalently-attached CDPI3 moieties have stephan erdman approach at will pdf DNA affinity and have improved the hybridization properties of sequence-specific DNA probes. DNA to give more stable DNA duplexes than unmodified oligonucleotides of similar length.
DNA probes with conjugated MGB groups form extremely stable duplexes with single-stranded DNA targets, allowing shorter probes to be used for hybridization based assays. MGB region of the duplex. MGB probes, therefore, can be significantly shorter than traditional probes, providing better sequence discrimination and flexibility to accommodate more targets. The simplest approach to MGB probe design is to use an MGB support, add a quencher molecule as the first addition and complete the synthesis with a 5′-fluorophore. Alternatively, a fluorophore support could be used with the 5′ terminus containing a quencher molecule followed by a final MGB addition at the 5′ terminus.
Specific instructions are provided for the use of these products and the procedures are illustrated using HPLC and MS data. In this review article, Young K. Errors during replication of damaged sites in DNA leads to mutations in genes and eventually cancer. The authors note that the detailed studies of the molecular mechanisms of DNA repair pathways were made possible by using site-specifically modified oligonucleotides and that the availability of phosphoramidites to synthesize oligonucleotides with DNA lesions has contributed to the field.
Phosphoramidites that allow the generation of oligonucleotides containing site-specific lesions have been vital components for studying the mechanism of DNA repair. New DNA lesions are still being discovered and the study of their biological consequences will require their site-specific incorporation into oligonucleotides. The authors conclude that the increased availability of phosphoramidites for the synthesis of lesion-containing oligonucleotides should facilitate many future discoveries in the broad area of DNA damage and repair. Indeed, ASGPR is the ideal target for delivery of therapeutic oligonucleotides to the liver since it combines tissue specificity, high expression levels and rapid internalization and turnover. Glen Research is delighted to introduce a GalNAc modification strategy using a monomeric GalNAc support and the equivalent GalNAc phosphoramidite.
Our experimental work has shown that these products are fully compatible with regular oligonucleotide synthesis and deprotection. Oligonucleotides containing GalNAc can be deprotected using standard procedures during which the acetyl protecting groups on the GalNAc group are removed. Glen Research offers these GalNAc C3 products under an agreement with AM Chemicals LLC. In this article, we review a variety of sequence modifiers that might be useful in the design of MGB probes containing a fluorophore, quencher, etc.